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PureYield™ Plasmid Midiprep System

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Description

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Catalog number:  A2492

Catalog number:  A2495

Catalog number:  A2496

The PureYield™ Plasmid Midiprep System provides a rapid method for purification of 100–200μg of transfection-quality plasmid DNA from 50ml bacteria cultures. Using the vacuum protocol, plasmid isolation is complete in as little as 30 minutes, significantly reducing time spent on purification compared to silica resin or other membrane-column methods. An alternative protocol allows purification of over 400μg of high-copy-number plasmid from 250ml of E. coli culture.

The system incorporates a unique Endotoxin Removal Wash designed to remove substantial amounts of protein, RNA and endotoxin contaminants from purified plasmid DNA. Removal of contaminants improves performance in sensitive applications such as eukaryotic transfection, in vitro transcription and coupled in vitro transcription/translation. Purification is achieved without isopropanol precipitation of purified plasmid DNA or extensive centrifugation.

PureYield™ Plasmid Midipreps can be used with centrifugation or vacuum-based protocols.

Proper assembly of Lysate Clearing Column (blue) and DNA Binding Column (white) for use with the PureYield™ Plasmid Midiprep System DNA Purification by Vacuum protocol.

Proper assembly of Lysate Clearing Column (blue) and DNA Binding Column (white) for use with the PureYield™ Plasmid Midiprep System DNA Purification by Vacuum protocol. Proper assembly of Lysate Clearing Column (blue) and DNA Binding Column (white) for use with the PureYield™ Plasmid Midiprep System DNA Purification by Vacuum protocol.

Protocols

Complete Protocol

PureYield™ Plasmid Midiprep System Technical Manual


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PureYield Plasmid Midiprep System Quick Protocol FB077
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What’s in the box?

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

What’s in the box?

[wpdatatable id=253 table_view=regular]

[ts-icon]

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

What’s in the box?

[wpdatatable id=254 table_view=regular]

[ts-icon]

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions