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PstI

  • Capable of digesting DNA in 15 minutes or less
  • Active and capable of digestion directly in GoTaq® Green Master Mix
  • Blue/white cloning qualified, provides a higher level of quality control for enzymes used in cloning applications

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Description

10u/μl

Catalog number selected: R6111

3,000u

Catalog number selected: R6111

15,000u

Catalog number selected: R6115

Cut Site:

C   TGCAG
GACGT   C

Notes:

  1. Star activity may be observed with glycerol concentrations >12% or with enzyme:DNA ratios >25u/μg.
  2. Ends generated by PstI can be directly ligated to ends generated by NsiI. Neither restriction site is regenerated in the ligation product.

Incubation Conditions: Buffer H. 37°C.

Source: Recombinant E. coli strain.

Storage Buffer: 10mM Tris-HCl (pH 7.3), 300mM NaCl, 0.1mM EDTA, 1mM DTT, 0.15% Triton® X-100, 0.5mg/ml BSA, 50% glycerol.

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Protocols

Complete Protocol

 

 Restriction Enzyme Protocol

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What’s in the box?

You are viewing: R6111 Change Configuration

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

What’s in the box?

You are viewing:  R6115 Change Configuration

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SDS

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions