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PolyATtract® mRNA Isolation Systems

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Description

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(with magnetic stand) MagneSphere® Technology Magnetic Separation Stand (2-position)

Catalog number: Z5200

(without magnetic stand)

Catalog number:  Z5210

(with magnetic stand) MagneSphere® Technology Magnetic Separation Stand (2-position)

Catalog number: Z5300

(without magnetic stand)

Catalog number:  Z5310

The PolyATtract® mRNA Isolation Systems use the MagneSphere® technology to isolate mRNA rapidly and effectively from total RNA. The systems use a biotinylated oligo(dT) primer to hybridize, at high efficiency in solution, to the 3´ poly(A)+ region present in most mature eukaryotic mRNAs. The hybrids are bound to streptavidin coupled to paramagnetic particles, captured using a magnetic separation stand and washed at high stringency. The highly pure mRNA is eluted from the solid phase by the simple addition of ribonuclease-free, deionized water. With total RNA as the starting material, poly(A)+ mRNA is isolated in approximately 45 minutes. The isolated mRNA is suitable for all molecular biology applications, including in vitro translation and cDNA synthesis.

Protocols

Complete Protocol

PolyATtract® mRNA Isolation Systems Technical Manual


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Quick Protocols

PolyATract mRNA Isolation Systems Quick Protocol


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What’s in the box?

(with magnetic stand) MagneSphere® Technology Magnetic Separation Stand (2-position)

[wpdatatable id=223 table_view=regular]

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

What’s in the box?

(without magnetic stand)

[wpdatatable id=224 table_view=regular]

[ts-icon]

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

What’s in the box?

(with magnetic stand) MagneSphere® Technology Magnetic Separation Stand (2-position)

[wpdatatable id=225 table_view=regular]

[ts-icon]

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

What’s in the box?

(without magnetic stand)

[wpdatatable id=226 table_view=regular]

[ts-icon]

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions