Molecular Products Co.

Search
customer-support

Call Us Now:

0334-7752577

pGEM®-T Easy Vector Systems

Convenience and Flexibility for Cloning PCR Products

  • Three options for removing the insert with a single digest (BstZI, EcoRI or NotI )
  • Ligation can be completed in 1 hour at room temperature
  • Available with or without competent cells

Please Enquire

Description

[ts-heading h2=”Size” css_animation=”fadeInUp”][/ts-heading][ts-custom-heading text=”PCR Cloning with Blue/White Selection and Easy Insert Excision” font_container=”tag:h1|text_align:center” css_animation=”fadeIn”]

The pGEM®-T Easy Vector Systems are convenient systems to clone PCR products generated by certain thermostable polymerases. These polymerases often add a single deoxyadenosine, in a template-independent fashion, to the 3´-ends of the amplified fragments. The pGEM®-T Easy pre-linearized Vector contains 3´-T overhangs at the insertion site to provide a compatible overhang for PCR products. The provided 2X Rapid Ligation Buffer allows reactions to be completed in 1 hour at room temperature. The incubation period may be extended to increase the number of colonies after transformation.

T7 and SP6 RNA polymerase promoters flank a multiple cloning region within the α-peptide coding region for β-galactosidase. Insertional inactivation of the α-peptide allows recombinant clones to be directly identified by Blue/White Screening on indicator plates. The vector allows preparation of single-stranded DNA due to its f1 Origin of Replication.

The pGEM®-T Easy Vector Systems offer all of the advantages of the pGEM®-T Vector Systems with the added convenience of recognition sites for BstZI, EcoRI and NotI flanking the insertion site. Thus, several options exist to remove the desired insert DNA with a single restriction digestion.

The pGEM®-T Easy Vector System II contains JM109 Competent Cells in addition to all of the pGEM®-T Easy Vector System I components.

There is no GenBank® Accession Number for the pGEM®-T Easy Vector. The parent vector is linearized at the position indicated by * in this pGEM®-T Easy Vector Sequence and a “T” is added at each end.

Protocols

Complete Protocol

pGEM®-T and pGEM®-T Easy Vector Systems Technical Manual

[easy_media_download url=”https://gomolecular.pk/wp-content/uploads/2021/06/pGEM-T-and-pGEM-T-Easy-Vector-Systems-TM042.zip” text=”Download” color=”blue_three” ]

Quick Protocols

pGEM T and pGEM T Easy Vector Systems FB033

[easy_media_download url=”https://gomolecular.pk/wp-content/uploads/2021/06/pGEM-T-and-pGEM-T-Easy-Vector-Systems-FB033.zip” text=”Download” color=”blue_three” ]

[ts-heading h2=”Specifications” txt_align=”center” css_animation=”fadeInUp”][/ts-heading]

What’s in the box?

[wpdatatable id=68 table_view=regular]

SDS For A1360

[easy_media_download url=”https://gomolecular.pk/wp-content/uploads/2021/06/A1360.zip” text=”Download SDS” color=”blue_three” ]

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

See Protocol for detailed storage recommendations.

What’s in the box?

[wpdatatable id=69 table_view=regular]

SDS For  A1380

[easy_media_download url=”https://gomolecular.pk/wp-content/uploads/2021/06/A1380.zip” text=”Download SDS” color=”blue_three” ]

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

See Protocol for detailed storage recommendations.