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pGEM®-7Zf(+/–) Vectors

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Description

Choose a vector

pGEM®-7Zf(–) Vector
Size: 20μg
Catalog number selected:  P2371

pGEM®-7Zf(+) Vector
Size: 20μg
Catalog number selected:  P2251

The pGEM®-7Zf(+) and pGEM®-7Zf(–) Vectors contain SP6 and T7 RNA polymerase promoters flanking a region of multiple cloning sites within the α-peptide coding region of β-galactosidase. This arrangement of promoters means both sense and antisense RNA can be produced. Insertional inactivation of the α-peptide identified recombinant clones by color screening on indicator plates when using appropriate E. coli strains (e.g., JM109). The multiple cloning region is unique and includes restriction sites for ApaI, AatII, SphI, XbaI, XhoI, EcoRI, KpnI, SmaI, ClaI, HindIII, BamHI, SacI, BstXI and NsiI. pGEM®-7Zf(+) and pGEM®-7Zf(–) Vectors are identical except for the orientation of the f1 origin.

Reference

  1. Yanisch-Perron, C. et al. (1985) Gene 33, 103–19.
0286VC-W

pGEM®-7Zf(+) Vector GenBank® Accession Number X65310 and vector sequence text file.

 

0286VB02_5A-W

pGEM®-7Zf(–) Vector GenBank® Accession Number X65311 and vector sequence text file.

Protocols

Complete Protocol

pGEM®-7Zf(+) Vector Technical Bulletin

 

Download now

pGEM®-7Zf(–) Vector Technical Bulletin

 

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What’s in the box?

Catalog number : P2371

Item Part # Size
pGEM®-7Zf(–) Vector P237A 1 × 20μg

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

See Protocol for storage recommendations.

What’s in the box?

Catalog number : P2251

Item Part # Size
pGEM®-7Zf(+) Vector P225A 1 × 20μg
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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

See Protocol for storage recommendations.