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pGEM®-5Zf(+) Vector

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Description

20μg
Catalog number selected: P2241

The pGEM®-5Zf(+) Vector serves as a standard cloning vector and as a template for in vitro transcription. This vector contains T7 and SP6 RNA polymerase promoters flanking a multiple cloning region within the α-peptide coding region of β-galactosidase. Insertional inactivation of the α-peptide allows recombinant clones to be identified directly by color screening on indicator plates when using appropriate E. coli strains (e.g., JM109). The multiple cloning region contains unique restriction sites for ApaI, AatII, SphI, NcoI, SacII, EcoRV, SpeI, NotI, PstI, SalI, NdeI, SacI, BstXI and NsiI.

Reference

  1. Yanisch-Perron, C. et al. (1985) Gene 33, 103–19.
0284VC02_5A-W

pGEM®-5Zf(+) Vector  GenBank® Accession Number X65308 and vector sequence text file.

Protocols

Complete Protocol

pGEM®-5Zf(+) Vector Technical Bulletin

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What’s in the box?

Item Part # Size
pGEM®-5Zf(+) Vector P224A 1 × 20μg

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB