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pGEM®-3Zf(+/–) Vectors

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Description

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pGEM®-3Zf(–) Vector
Size: 20μg
Catalog number selected: P2261

pGEM®-3Zf(+) Vector
Size: 20μg
Catalog number selected: P2271

The pGEM®-3Zf(+) and pGEM®-3Zf(–) Vectors contain T7 and SP6 RNA polymerase promoters flanking a multiple cloning region within the α-peptide coding region of β-galactosidase. Insertional inactivation of the α-peptide allows recombinant clones to be identified directly by color screening on indicator plates when using appropriate E. coli strains (e.g., JM109). The multiple cloning region contains unique restriction sites for EcoRI, SacI, KpnI, AvaI, SmaI, BamHI, XbaI, SalI, AccI, HincII, PstI, SphI and HindIII. The pGEM®-3Zf(+) and -3Zf(–) Vectors can be used as standard cloning vectors and as templates for in vitro transcription.

Reference

  1. Yanisch-Perron, C. et al. (1985) Gene 33, 103–19.
0282VC05_4A-W

pGEM®-3Zf(+) Vector GenBank® Accession Number X65306 and vector sequence text file.

0282VB05_4A-W

pGEM®-3Zf(–) Vector GenBank® Accession Number X65307 and vector sequence text file.

Protocols

Complete Protocol

pGEM®-3Zf(+) Vector Technical Bulletin


Download now

pGEM®-3Zf(–) Vector Technical Bulletin


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What’s in the box?

Catalog number : P2261

Item Part # Size
pGEM®-3Zf(–) Vector P226A 1 × 20μg

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

See Protocol for storage recommendations.

What’s in the box?

Catalog number : P2271

Item Part # Size
pGEM®-3Zf(+) Vector P227A 1 × 20μg
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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

See Protocol for storage recommendations.