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pFN2 (GST) Flexi® Vectors

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Description

Choose an antibiotic resistance

Size: 20μg
Catalog number:   C8461

Size: 20μg
Catalog number:  C8471

The pFN2A and pFN2K (GST) Flexi® Vectors are used for inducible expression of GST-tagged fusion proteins in E. coli and cell-free systems using the T7 RNA polymerase promoter. The GST tag has been successfully used to boost tagged protein solubility during E. coli expression. Reusable gluthathione (GST-affinity) resins can be used with the pFN2 (GST) Flexi® Vectors for standard protein purification and prokaryotic expression applications. Express cloned GST-tagged ORFs in bacterial and cell-free systems.

About the Flexi® Vector System

The Flexi® Vector System is a simple, yet powerful, directional cloning method for protein-coding sequences. It is based on two rare-cutting restriction enzymes, SgfI and PmeI, and provides a rapid, efficient and high-fidelity way to transfer protein-coding regions between a variety of Flexi® Vectors without the need to resequence. Directly use recombinant clones and minimize time wasted screening background colonies. The versatile cloning of the Flexi® system means you can choose from a variety of expression systems and fusion tag orientations and then transfer to others as required. Direct transfers can only occur between two N-terminal tagged vectors or from an N-terminal to a C-terminal vector.

Note: Flexi® Vectors contain the lethal barnase gene to reduce background colonies without inserts during the subcloning procedure. Using the Flexi® Vector Cloning System replaces the barnase gene with your insert. These vectors, as purchased, cannot be cultured in normal laboratory strains of E. coli without an insert.

Sequence Information

pFN2A (GST) Flexi® Vector GenBank® Accession Number AY753578 and vector sequence text file.

pFN2K (GST) Flexi® Vector GenBank® Accession Number AY753579 and vector sequence text file.

Protocols

Complete Protocol

pFN2A (GST) Flexi® Vector Product Information


pFN2K (GST) Flexi® Vector Product Information
[ts-heading h2=”Specifications” txt_align=”center” css_animation=”fadeInUp”][/ts-heading]
Item /catlog no: C8461 Part # Size
pFN2A (GST) Flexi® Vector C846A 1 × 20μg

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

 

Item /catlog no: C8471 Part # Size
pFN2K (GST) Flexi® Vector C847A 1 × 20μg

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

 

Patents and Disclaimers

U.S. Pat. Nos. 8,293,503, 9,018,014 and 8,367,403, European Pat. No. 1685247 and other patents and patents pending.

Patent Pending.

For research use only. Persons wishing to use this product or its derivatives in other fields of use, including without limitation, commercial sale, diagnostics or therapeutics, should contact Promega Corporation for licensing information.