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pFC14A and pFC14K HaloTag® CMV Flexi® Vectors

Appends HaloTag® to C-Terminus of Protein Fusion Partner

  • Enables constitutive protein expression in mammalian cells
  • Includes CMV immediate-early enhancer/promoter
  • Suited to stable or transient gene expression

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Size: 20µg
Catalog number: G9651

Size: 20µg
Catalog number: G9661

Constitutive Expression in Mammalian Cells

The pFC14A and pFC14K HaloTag® CMV Flexi® Vector are configured to append HaloTag® to the carboxyterminus of the protein fusion partner and provide constitutive protein expression in mammalian cells using the human cytomegalovirus (CMV) immediate early enhancer/promoter. The vector can be used for both stable and transient gene expression; for stable expression, cotransfection with a vector containing a selectable marker is required.

Note: Flexi® Vectors contain the lethal barnase gene to reduce background colonies without inserts during the subcloning procedure. Using the Flexi® Vector Cloning System replaces the barnase gene with your insert. These vectors, as purchased, cannot be cultured in normal laboratory strains of E. coli without an insert.


  • Clone ORF and conveniently transfer these inserts to a variety of vectors designed for multiple applications.

GenBank® Accession Numbers and Sequence Information

pFC14A HaloTag® CMV GenBank® Accession Number EU113046; pFC14A HaloTag® CMV Vector sequence text file.
pFC14K HaloTag® CMV GenBank® Accession Number EU113047; pFC14K HaloTag® CMV Vector sequence text file.


Complete Protocol

pFC14A HaloTag® CMV Flexi® Vector Protocol

pFC14K HaloTag® CMV Flexi® Vector Protocol
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Item /catlog no:  G9651 Part # Size
pFC14A HaloTag® CMV Flexi® Vector G965A 1 × 20μg

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions


Item/ catlog no: G9661 Part # Size
pFC14K HaloTag® CMV Flexi® Vector G966A 1 × 20μg

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions


Patents and Disclaimers

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE STATEMENT. If the researcher is not willing to accept the conditions of this limited use statement, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund. Researchers may use this product for research use only, no commercial use is allowed. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the HaloTag® gene. Researchers may however clone heterologous DNA sequences at either or both ends of said HaloTag® gene so as to create fused gene sequences provided that the coding sequence of the resulting HaloTag® gene has no more than four (4) deoxynucleotides missing at the affected terminus when compared to the intact HaloTag® gene sequence. In addition, researchers must do one of the following in conjunction with use of the product: (1) use Promega HaloTag® ligands, which can be modified or linked to Promega or customer-supplied moieties, or (2) contact Promega to obtain a license if Promega HaloTag® ligands are not to be used. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this agreement shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. Nos. 8,293,503, 9,018,014 and 8,367,403, European Pat. No. 1685247 and other patents and patents pending.

U.S. Pat. Nos. 7,425,436, 7,935,803, 8,466,269, 8,742,086, 8,420,367, 8,748,148, 9,416,353, 9,593,316 and other patents and patents pending.

For research use only. Persons wishing to use this product or its derivatives in other fields of use, including without limitation, commercial sale, diagnostics or therapeutics, should contact Promega Corporation for licensing information.