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pF3 WG (BYDV) Flexi® Vectors

Protein Expression Vectors for use with Wheat Germ Cell-Free Systems

  • Cloned inserts are in vitro translated in WGE systems
  • Adaptable to high-throughput formats for large screening projects
  • Transfer your cloned insert into other Flexi® Vectors for additional applications (e.g., bacterial or mammalian protein expression) as needed

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Description

Choose an antibiotic resistance

Size: 20µg
Catalog number: L5671

Size: 20µg
Catalog number: L5681

The pF3A and pF3K WG (BYDV) Flexi® Vectors were designed for use with Wheat Germ extracts and contain sequences from the barley yellow dwarf virus (BYDV), an RNA plant virus, upstream and downstream of the protein coding region of interest. The BYDV elements interact with each other, form a closed loop and act synergistically to stimulate translation in wheat germ extracts, bypassing mRNA cap and polyadenylation dependencies. These vectors need no licensing fees or complicated transfer restrictions to use. Just clone your ORFs and conveniently transfer the inserts to a variety of vectors designed for multiple applications.

About the Flexi® Vector System

The Flexi® Vector System is a simple, yet powerful, directional cloning method for protein-coding sequences. It is based on two rare-cutting restriction enzymes, SgfI and PmeI, and provides a rapid, efficient and high-fidelity way to transfer protein-coding regions between a variety of Flexi® Vectors without the need to resequence. These in vitro translation Flexi® vectors are designed with special sequences for maximal cell-free protein expression in a specific extract system.

Note: Flexi® Vectors contain the lethal barnase gene to reduce background colonies without inserts during the subcloning procedure. Using the Flexi® Vector Cloning System replaces the barnase gene with your insert. These vectors, as purchased, cannot be cultured in normal laboratory strains of E. coli without an insert.

Ready-to-Use HaloTag® Flexi® Vector Clones

For access to 20,000 human and 15,000 mouse ORF clones with HaloTag® Technology in multiple mammalian expression vectors and custom cloning options, visit: www.kasuza.or.jp.kop/dsearch-e

 

Wheat Germ BYDV Vector
 

pF3A WG (BYDV) Flexi® Vector GenBank® Accession Number AY949043 and vector sequence text file.

 

Wheat Germ BYDV protein expression vector pF3K
 

pF3K WG (BYDV) Flexi® Vector GenBank® Accession Number AY949044 and vector sequence text file.

Protocols

Complete Protocol

pF3A WG (BYDV) Flexi® Vector

 

pF3K WG (BYDV) Flexi® Vector
[ts-heading h2=”Specifications” txt_align=”center” css_animation=”fadeInUp”][/ts-heading]
Item Part # Size
pF3A WG (BYDV) Flexi® Vector L567A 1 × 20μg

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Item Part # Size
pF3K WG (BYDV) Flexi® Vector L568A 1 × 20μg

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. Nos. 8,293,503, 9,018,014 and 8,367,403, European Pat. No. 1685247 and other patents and patents pending.

Patent Pending.

For research use only. Persons wishing to use this product or its derivatives in other fields of use, including without limitation, commercial sale, diagnostics or therapeutics, should contact Promega Corporation for licensing information.

Licensed under U.S. Pat. No. 5,910,628.