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pF25 ICE T7 Flexi® Vectors

Protein Expression Vectors Used With Insect Cell Extracts for In Vitro Translation

  • Efficient transfer of Flexi® vector inserts for direct use of recombinant clones, minimizing time wasted screening background colonies
  • Adaptable to high-throughput formats for large screening projects
  • Transfer your cloned insert into other Flexi® Vectors for additional applications (e.g., bacterial or mammalian protein expression) as needed

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Choose an antibiotic resistance

Size: 20µg
Catalog number: L1061

Size: 20µg
Catalog number: L1081

The pF25A and pF25K ICE T7 Flexi® Vectors were designed for use with Insect Cell Extracts and contain untranslated region (UTR) sequences at the 5´ and 3´ ends of the gene coding region to enhance translation efficiency. These vectors need no licensing fees or complicated transfer restrictions to use. Just clone your ORFs and conveniently transfer the inserts to a variety of other Flexi® vectors designed for multiple applications.

About the Flexi® Vector System

The Flexi® Vector System is a simple, yet powerful, directional cloning method for protein-coding sequences. It is based on two rare-cutting restriction enzymes, SgfI and PmeI, and provides a rapid, efficient and high-fidelity way to transfer protein-coding regions between a variety of Flexi® Vectors without the need to resequence. These in vitro translation Flexi® vectors are designed with special sequences for maximal cell-free protein expression in a specific extract system.

Note: Flexi® Vectors contain the lethal barnase gene to reduce background colonies without inserts during the subcloning procedure. Using the Flexi® Vector Cloning System replaces the barnase gene with your insert. These vectors, as purchased, cannot be cultured in normal laboratory strains of E. coli without an insert.

Ready-to-Use HaloTag® Flexi® Vector Clones

For access to 20,000 human and 15,000 mouse ORF clones with HaloTag® Technology in multiple mammalian expression vectors and custom cloning options, visit:

Sequence Information

pF25A ICE T7 Flexi® Vector GenBank® Accession Number EU754721 and vector sequence text file.

pF25K ICE T7 Flexi® Vector GenBank® Accession Number EU754722 and vector sequence text file.


Complete Protocol

pF25A ICE T7 Flexi® Vector


pF25K ICE T7 Flexi® Vector
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Item Part # Size
pF25A ICE T7 Flexi® Vector L106A 1 × 20μg

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions


Item Part # Size
pF25K ICE T7 Flexi® Vector L108A 1 × 20μg

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions


Patents and Disclaimers

U.S. Pat. Nos. 8,293,503, 9,018,014 and 8,367,403, European Pat. No. 1685247 and other patents and patents pending.

Patent Pending.

For research use only. Persons wishing to use this product or its derivatives in other fields of use, including without limitation, commercial sale, diagnostics or therapeutics, should contact Promega Corporation for licensing information.

Ezure, T., Suzuki, T., Higashide, S., Shintani, E., Endo, K., Kobayashi, S., Shikata, M., Ito, M., Tanimizu, K., Nishimura, O. (2006) Cell-free protein synthesis system prepared from insect cells by freeze-thawing. Biotechnol. Prog. 22, 1570–7.