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pF1 T7 Flexi® Vectors

Untagged Bacterial Expression Vectors

  • Generate protein from T7 promoter in E. coli and cell-free expression systems
  • Options for Kanamycin and Ampicillin resistance
  • Starting vector for transferring ORFs into other Flexi® Vectors

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Description

Choose an antibiotic resistance

Size: 20μg
Catalog number:  C8441

Size: 20μg
Catalog number: C8451

F1A and pF1K T7 Flexi® Vectors are designed for untagged protein expression in E. coli and cell-free systems using the T7 RNA polymerase promoter. Expression levels depend highly on the nature of the protein.

About the Flexi® Vector System

The Flexi® Vector System is a simple, yet powerful, directional cloning method for protein-coding sequences. It is based on two rare-cutting restriction enzymes, SgfI and PmeI, and provides a rapid, efficient and high-fidelity way to transfer protein-coding regions between a variety of Flexi® Vectors without the need to resequence. Directly use recombinant clones and minimize time wasted screening background colonies. The versatile cloning of the Flexi® system means you can choose from a variety of expression systems and fusion tag orientations and then transfer to others as required. Direct transfers can only occur between two N-terminal tagged vectors or from an N-terminal to a C-terminal vector.

Note: Flexi® Vectors contain the lethal barnase gene to reduce background colonies without inserts during the subcloning procedure. Using the Flexi® Vector Cloning System replaces the barnase gene with your insert. These vectors, as purchased, cannot be cultured in normal laboratory strains of E. coli without an insert.

Sequence Information

pF1A T7 Flexi® Vector GenBank® Accession Number AY753576 and vector sequence text file.

pF1K T7 Flexi® Vector GenBank® Accession Number AY753577 and vector sequence text file.

Protocols

Complete Protocol

pF1A T7 Flexi® Vector Product Information

 

pF1K T7 Flexi® Vector Product Information
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Item /catlog no: C8441 Part # Size
pF1A T7 Flexi® Vector C844A 1 × 20μg

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

 

Item /catlog no: C8451 Part # Size
pF1K T7 Flexi® Vector C845A 1 × 20μg

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

 

Patents and Disclaimers

U.S. Pat. Nos. 8,293,503, 9,018,014 and 8,367,403, European Pat. No. 1685247 and other patents and patents pending.

Patent Pending.

For research use only. Persons wishing to use this product or its derivatives in other fields of use, including without limitation, commercial sale, diagnostics or therapeutics, should contact Promega Corporation for licensing information.