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NheI

  • Capable of digesting DNA in 15 minutes or less
  • Active and capable of digestion directly in GoTaq® Green Master Mix
  • Genome qualified to ensure optimal performance in genomic analysis applications

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Description

10μ/μl

Catalog number selected: R6501

250u

Catalog number selected: R6501

1,250u

Catalog number selected: R6505

Cut Site:

GCTAG   C
C   GATCG

Notes:

  1. For site-specific methylation information, consult McClelland, M., Nelson, M. and Raschke, E. (1994) Nucl. Acids Res. 22, 3640–59.
  2. Ends generated with NheI can be directly ligated to ends generated by SpeI or XbaI. None of these recognition sites are regenerated in the ligation product.

Incubation Conditions: Buffer B. 37°C.

Source: Neisseria mucosa heidelbergensis.

Storage Buffer: 10mM Tris-HCl (pH 7.4), 50mM NaCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.

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Protocols

Complete Protocol

 

 Restriction Enzyme Protocol

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What’s in the box?

You are viewing: R6505 Change Configuration

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SDS

 

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

What’s in the box?

You are viewing: R6501 Change Configuration

[wpdatatable id=444 table_view=regular]

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions