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NdeI

  • Capable of digesting DNA in 15 minutes or less
  • Active and capable of digestion directly in GoTaq® Green Master Mix

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Description

10μ/μl

Catalog number selected: R6801

500u

Catalog number selected: R6801

Cut Site:

CATA  TG
GT   ATAC

Notes:

  1. Star activity may be observed with glycerol concentrations >10%.
  2. For site-specific methylation information, consult McClelland, M., Nelson, M. and Raschke, E. (1994) Nucl. Acids Res. 22, 3640–59.
  3. Ends generated with NdeI can be directly ligated to ends generated by VspI. Neither recognition site is regenerated in the ligation product.

Incubation Conditions: Buffer D. 37°C.

Source: Neisseria denitrificans.

Storage Buffer: 10mM Tris-HCl (pH 7.4), 300mM NaCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.

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Protocols

Complete Protocol

 

 Restriction Enzyme Protocol

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What’s in the box?

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions