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Maxwell® RSC miRNA from Tissue and Plasma or Serum

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Description

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Size: 48 preps

Catalog number: AS1680

Size: 48 preps

Catalog number:  AS1460

Automated Purification of High-Quality Total RNA with an Enhanced miRNA Enrichment

 

 

 

 

 

Purify High-Quality miRNA from Tissue, Plasma, Serum or Enriched Exosomes

The Maxwell® RSC miRNA Tissue Kit purifies total RNA, including microRNA (miRNA), from 1–48 tissue samples in about 90 minutes. The Maxwell® RSC miRNA Plasma and Serum Kit will purify total RNA, including miRNA, from 1–48 samples in about 70 minutes. The kits provide all the reagents required for sample processing and use prefilled cartridges for purification, simplifying extraction and freeing up your time for analysis.

The Maxwell® RSC miRNA Tissue and miRNA Plasma and Serum Kits work with both the Maxwell® RSC and RSC 48 instruments. The Maxwell® RSC Instrument can process from 1 to 16 samples and the Maxwell® RSC 48 can process from 1 to 48 samples in a single run. The Maxwell® RSC miRNA Tissue and miRNA Plasma and Serum Kits are also compatible for use on the Maxwell® CSC Instrument in RUO Mode.

The extracted RNA is highly concentrated and ready for use in RT-qPCR, RNA sequencing and other downstream applications used in biomarker detection, gene expression and other studies.

No clogging worries with a paramagnetic particle mover

Because the Maxwell® RSC Instruments are magnetic particle movers, not liquid handlers, they offer advantages over other automated systems. There is minimal risk of cross-contamination because no liquid handling of splattering happens during sample processing. With no clogs and fewer breakdowns, there are fewer disruptions in your workflow.

[ts-heading h2=”Simple, safe and efficient protocol” txt_align=”center” css_animation=”fadeInUp”]Purify RNA that is ready for use in downstream assays with minimal preprocessing and no organic reagents.[/ts-heading]

Using tissue

Automated miRNA extraction from tissue protocol

Using plasma, serum or enriched exosomes

Automated miRNA extraction from plasma serum or exosomes protocol
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miRNA from tissue

Multiple miRNA targets can be consistently purified from mammalian tissues. The high-quality RNA is ready to use in downstream amplification assays such as RT-qPCR.

high yields of automated miRNA extraction from tissue

high yields of automated miRNA extraction from different tissues


miRNA purified from pancreas, kidney and liver samples.
miRNA yield was quantitated for both mir-21 and let-7a targets by RT-qPCR using TaqMan®-based miRNA-specific primers and standards.

miRNA from plasma

Consistent purification of high-quality miRNA from plasma, serum or enriched exosomes.

 

high yields of automated miRNA extraction from plasma
miRNA purified from plasma. Comparison of the total yield of miR-21 purified from 200μl of plasma using the Maxwell® RSC miRNA Plasma and Serum Kit or the miRNeasy Serum/Plasma Kit (Qiagen). N = 4.

Protocols

Complete Protocol

Maxwell® RSC miRNA Tissue Kit Technical Manual

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Maxwell® RSC miRNA Plasma and Serum Kit Technical Manual

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Quick Protocols

Maxwell RSC miRNA Tissue Kit FB188

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Maxwell® RSC miRNA Plasma and Serum Kit Quick Protocol

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What’s in the box?
Size: 48 preps

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

What’s in the box?
Size: 48 preps

[wpdatatable id=199 table_view=regular]

[ts-icon]

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

Patents and Disclaimers

U.S. Pat. No. 7,329,488 and S. Korean Pat. No. 100483684.