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Maxwell® HT Viral TNA Kit, Custom

High-Throughput Extraction of RNA and DNA from Viral Samples

  • Robust protocol supports various viral sample types and a range of input volumes
  • Magnetic separation avoids the use of solvents
  • Ideal for use in downstream applications such as qPCR

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Description

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Automated Purification of Total Nucleic Acid from Viral Sample Types

Emergence of new viral diseases, including the coronavirus SARS-CoV-2 (COVID 19), MERS (2015) and SARS (2003) outbreaks, highlight the need for fast methods to detect and identify target viruses at scale. The Maxwell® HT Viral TNA Kit can be used for high-throughput, automated extraction of RNA and DNA from several sample types, including universal transport medium, serum, plasma and others. Samples are processed in 96-well plates using magnetic separation. The Maxwell® HT purification kit can also be used in manual protocols using a multichannel pipettor.

Protocols are available for automated platforms and manual use. These are provided in the Application Notes linked below.

Inactivated HIV-1 (5,000IU/ml) and 500IU/ml of inactivated Hepatitis B virus (HBV) were isolated from a 300μl sample of human plasma using the Maxwell® HT Viral TNA chemistry automated on a Tecan Freedom EVO® and compared with performance of the Qiagen QIAamp® MinElute® kit. Quantitation was conducted using PathID™ RT-qPCR (HIV-1) and Plexor® RT-qPCR (HBV).

Maxwell® HT RNA and DNA Extraction Protocol Overview

Viral and bacterial RNA and DNA isolation with Maxwell HT protocol
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What’s in the box?

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Use Restrictions

For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.

Storage Conditions

Patents and Disclaimers

U.S. Pat. No. 7,329,488 and S. Korean Pat. No. 100483684.