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Maxwell® 16 LEV Plant RNA Kit

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Description

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Catalog number: AS1430

Automated RNA Extraction from 1–16 Plant Tissue Samples in 60 Minutes

 

 

 

 

 

Faster RNA Purification, Less Hands-On Time and More Efficient Workflows

The Maxwell® 16 LEV Plant RNA Kit is used with the Maxwell® 16 Research Instrument (Cat.# AS2000) to provide an easy method for efficient, automated purification of RNA from plant tissue samples. The Maxwell® 16 Instrument is supplied with preprogrammed purification methods and is designed for use with the predispensed reagent cartridges supplied in the Maxwell® 16 LEV Plant RNA Kit. The instrument can process from 1 to 16 samples in under an hour, and the purified RNA can be used directly in downstream applications including real-time RT-qPCR, gel electrophoresis, microarrays and sequencing (e.g., NGS and Sanger).

  • Consistent processing and higher yields mean less re-isolation of samples, reducing costs and increasing the reliability of your results.
  • No organic extraction and minimal preprocessing required.
[ts-heading h2=”Purifies High-Quality RNA from Arabidopsis, Soybean and Corn” txt_align=”center” css_animation=”fadeInUp”][/ts-heading]
RNA was extracted from 40mg of Arabidopsis, corn and soybean leaf tissues (n=6 for each sample type) using the Maxwell® RSC Plant RNA Kit and eluted in 50µl. Eluted RNA was quantified using the QuantiFluor® RNA Dye and the Quantus™ Fluorometer.
RNA was extracted from 40mg of fresh leaf tissues (n=4 for each sample type) using the Maxwell® 16 Plant RNA kit and eluted in 50µl. Yield was determined by spectroscopy. A260/A280 ratios above 1.8, and A260/A230 ratios between 1.8 and 2.4 were achieved for all samples.
[ts-heading h2=”Purified RNA Performs Well In RT-PCR” txt_align=”center” css_animation=”fadeInUp”][/ts-heading]

RNA was extracted from 40mg of plant leaf tissue from the indicated species, eluted in 50µl and quantitated by RT-qPCR using species-specific primer/probe sets. The 1:10 dilution for all three species indicates that there is no inhibition based on volume input.

Inhibition of amplification of extracted RNA: Comparison to an Internal Positive Control. RNA samples (n=4), purified from leaves of the indicated plants, were added to a TaqMan® Exogenous Internal Positive Control (IPC) reaction. A ΔCt value of zero indicates no inhibition, and a ΔCt of two or below is considered acceptable. The observed ΔCt values for the Maxwell® 16 LEV Plant RNA samples indicate minimal inhibition.

Protocols

Complete Protocol

Maxwell® 16 LEV Plant RNA Kit Technical Manual

 

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Quick Protocols

Maxwell® 16 LEV Plant RNA Kit Quick Protocol, FB170


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What’s in the box?

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

Patents and Disclaimers

U.S. Pat. No. 6,855,499 and other patents.