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M-MLV Reverse Transcriptase, RNase H Minus, Point Mutant

High Yields of cDNA from Long RNA Templates (>5kb)

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Description

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Catalog number selected:  M3681

Catalog number selected: M3682

Catalog number selected: M3683

High Yields of cDNA from Long RNA Templates (>5kb)

Obtain Higher Yields of cDNA than with the Deletion Mutant

Moloney Murine Leukemia Virus Reverse Transcriptase, RNase H Minus (M-MLV RT [H–]), Point Mutant, is an RNA-dependent DNA polymerase that can be used in cDNA synthesis with long RNA templates (>5kb). The lack of RNase H activity is beneficial for this application, as RNase H can start to degrade templates when incubation times are long, as they may be when synthesizing long cDNAs. Although many researchers are successful in using M-MLV RT (H+) for analytical and some preparative cDNA applications, reverse transcriptases lacking RNase H activity provide another option to prepare long cDNAs and libraries containing a high percentage of full-length cDNA.

Advantages

  • RNase H Minus: Provides optimal conditions to prepare full-length cDNA from long RNA templates.
  • Temperature Stability: Thermostability of this point mutant minimizes problems associated with secondary structure.
  • Increased Polymerase Activity: M-MLV RT (H–), Point Mutant, gives higher yields of cDNA compared with the deletion mutant (Cat.# M5301).
  • Provided with 5X Reaction Buffer: 250mM Tris-HCl (pH 8.3 at 25°C), 375mM KCl, 15mM MgCl2, 50mM DTT.
  • Broad Working Range: More tolerance to variations in enzyme and substrate concentrations means improved consistency in performance.

Applications

  • First-strand cDNA synthesis.
  • Primer extension.

Thermal stability and synthesis of large transcripts.

Thermal stability and synthesis of large transcripts.

Protocols

Complete Protocol

M-MLV Reverse Transcriptase, RNase H Minus, Point Mutant Protocol
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What’s in the box?

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SDS For M3681

[easy_media_download url=”https://gomolecular.pk/wp-content/uploads/2021/06/M3681.zip” text=”Download SDS” color=”blue_three” ]

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

What’s in the box?

[wpdatatable id=87 table_view=regular]

SDS For  M3682

[easy_media_download url=”https://gomolecular.pk/wp-content/uploads/2021/06/M3682.zip” text=”Download SDS” color=”blue_three” ]

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

What’s in the box?

[wpdatatable id=88 table_view=regular]

SDS For M3683

[easy_media_download url=”https://gomolecular.pk/wp-content/uploads/2021/06/M3683.zip” text=”Download SDS” color=”blue_three” ]

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions