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LigaFast™ Rapid DNA Ligation System

Ligate DNA Inserts into Plasmid Vectors in 15 Minutes or Less at Room Temperature

  • DNA inserts with blunt ends or 5´ or 3´ overhangs can be used for ligation
  • No purification of ligated DNA needed prior to heat-shock transformation in E. coli
  • Ligations performed using the LigaFast™ System are comparable to standard overnight ligations

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Description

Size

The LigaFast™ Rapid DNA Ligation System is designed for the efficient ligation of sticky-ended DNA inserts into plasmid vectors in just 5 minutes (blunt-ended inserts in as little as 15 minutes). Rapid ligation is based on the combination of T4 DNA Ligase with a unique 2X Rapid Ligation Buffer. The LigaFast™ System is designed to eliminate any further purification prior to transformation of ligated DNA. The specially formulated 2X Rapid Ligation Buffer requires no additional ATP or Mg2+ addition prior to use.

The LigaFast™ System is Blue/White Cloning Qualified, providing a higher level of quality control for enzymes used in cloning applications.

Storage Buffer: 10mM Tris-HCl (pH 7.4 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA and 50% glycerol.

QC Tests: Activity, SDS-PAGE/purity, DNase, endonuclease, RNase, blue/white cloning assay.

Unit Definition: 0.01 Weiss unit of T4 DNA Ligase is the amount of enzyme required to catalyze the ligation of greater than 95% of 1μg of λ/HindIII fragments at 16°C in 20 minutes.

Protocols

Complete Protocol

LigaFast™ Rapid DNA Ligation System Protocol


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Item Part # Size
2X Rapid Ligation Buffer C671A 1 × 200μl
T4 DNA Ligase M180A 1 × 100u

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

 

Item Part # Size
2X Rapid Ligation Buffer C671B 2 × 500μl
T4 DNA Ligase M180B 1 × 500u

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB