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KpnI

  • Capable of digesting DNA in 15 minutes or less
  • Active and capable of digestion directly in GoTaq® Green Master Mix
  • Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications

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Description

8-12μ/μl

Catalog number selected: R6341

2,500u

Catalog number selected: R6341

10,000u

Catalog number selected: R6345

Cut Site:

G   GTACC
CCATG   G

Usage Notes:

  1. Star activity may be observed with glycerol concentrations >12% or with enzyme:DNA ratios >25u/μg.
  2. KpnI recognizes the same DNA sequence but cleaves at a different nucleotide than its neoschizomer, Acc65I.
  3. KpnI is not sensitive to overlapping dcm methylation, unlike its neoschizomer, Acc65I.

Incubation Conditions: Buffer J. 37°C.

Source: Recombinant E. coli strain.

Storage Buffer: 10mM Tris-HCl (pH 7.4), 200mM KCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.

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Protocols

Complete Protocol

 

 Restriction Enzyme Protocol

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What’s in the box?

You are viewing: R6341 Change Configuration

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

What’s in the box?

You are viewing: R6345 Change Configuration

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SDS

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions