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I-PpoI (Intron-Encoded Endonuclease)

Genome qualified to ensure optimal performance in genomic analysis applications

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Description

8-12μ/μl

Catalog number selected:  R7103

250u

Catalog number selected:  R7103

Cut Site:

CTCTC    TTAAGGTAGC
GAGAGAATT   CCATCG

Note: I-PpoI recognizes rDNA repeats (approximately 200) in S. cerevisiae chromosome 12, generating 600bp, 400bp and 9kb fragments.

Incubation Conditions: I-PpoI Buffer. 37°C.

Source: I-PpoI is an intron-encoded endonuclease from Physarum polycephalum. The enzyme is produced from a recombinant E. coli strain.

Storage Buffer: 50mM Tris-HCl (pH 7.5), 0.01% NP-40, 50mM NaCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.

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Note: A precipitate may be observed after thawing. If detected, heat to 37°C for 3–5 minutes and mix to dissolve. The buffer may appear cloudy after heating; this will not affect the performance of the product.

Frequency of Cutting: No sites are present in any of the commonly used plasmids or vectors. One site is present in chromosome 12 of S. cerevisiae DNA.

Protocols

Complete Protocol

 

 Restriction Enzyme Protocol

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What’s in the box?

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions