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GoTaq® Master Mixes

All-in-One Optimized PCR Master Mix—Just Add Template, Primers and Water

  • Directly substitute into your current PCR conditions
  • Use the green master mix for direct-to-gel analysis after amplification
  • Use the colorless master mix for post-amplification analysis by fluorescence or absorbance without prior DNA purification

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Description

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Size:

100 reactions: Catalog number selected: M7132

1000 reactions: Catalog number selected: M7133

Size:

100 reactions: Catalog number selected: M7122

1000 reactions: Catalog number selected: M7123

 

All-in-One Optimized PCR Master Mix—Just Add Template, Primers and Water

 

Set Up PCR in Less than a Minute with Convenient Master Mixes

GoTaq® DNA Polymerase is a proprietary formulation of Taq DNA polymerase that gives robust amplification equal to and, in some cases, superior to that of standard Taq DNA polymerase.

For ultimate in convenience, choose GoTaq® Colorless Master Mix or GoTaq® Green Master Mix and directly substitute in your current PCR application—no need to change cycling parameters.

Both Master Mixes are ready-to-use 2X solutions that contain GoTaq® DNA Polymerase, dNTPs, MgCl2 and reaction buffer at optimal concentrations for efficient amplification of DNA templates by PCR. GoTaq® Green Master Mix also includes two dyes (blue and yellow) that allow monitoring of progress during electrophoresis. The blue dye comigrates at the same rate as 3–5kb DNA fragments in a 1% agarose gel. The yellow dye migrates ahead of primers (<50bp).

GoTaq® Colorless Master Mix has the same formulation as the GoTaq® Green Master Mix but does not include the dyes. Both also include Nuclease-Free Water. Reactions assembled with the GoTaq® Master Mixes have sufficient density for direct loading onto agarose gels.

Reactions can be set up in less than a minute; simply add your template, water and primers and go!

GoTaq® Green and Colorless Master Mix reaction size is based on a 50µl reaction volume.

Protocols

Complete Protocol

GoTaq® Green Master Mix (M712) Protocol

[easy_media_download url=”https://gomolecular.pk/wp-content/uploads/2021/06/GoTaq-Green-Master-Mix-9PIM712.zip” text=”Download” color=”blue_three” ]

 

GoTaq® Colorless Master Mix (M713) Product

[easy_media_download url=”https://gomolecular.pk/wp-content/uploads/2021/06/GoTaq-Colorless-Master-Mix-9PIM713.zip” text=”Download” color=”blue_three” ]

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What’s in the box?

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SDS For M7132

[easy_media_download url=”https://gomolecular.pk/wp-content/uploads/2021/06/M7132.zip” text=”Download SDS” color=”blue_three” ]

 

SDS For M7133

[easy_media_download url=”https://gomolecular.pk/wp-content/uploads/2021/06/M7133.zip” text=”Download SDS” color=”blue_three” ]

Use Restrictions

For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.

Storage Conditions

What’s in the box?

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SDS For M7122

[easy_media_download url=”https://gomolecular.pk/wp-content/uploads/2021/06/M7122.zip” text=”Download SDS” color=”blue_three” ]

 

SDS For M7123

[easy_media_download url=”https://gomolecular.pk/wp-content/uploads/2021/06/M7123.zip” text=”Download SDS” color=”blue_three” ]

Use Restrictions

For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.

Storage Conditions

Patent and Disclaimers

Use of this product for basic PCR is outside of any valid US patents assigned to Hoffman La-Roche or Applera. This product can be used for basic PCR in research, commercial or diagnostic applications without any license or royalty fees.