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GoTaq® Hot Start Polymerase

Robust Hot Start PCR with Convenient Room-Temperature Setup

  • Hot Start Taq minimizes nonspecific amplification and primer-dimers
  • Optimize reaction conditions with supplied Flexi Reaction Buffer and MgCl2 solution
  • Direct-to-gel convenience
  • Supplied at a concentration of 5u/µl

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Description

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Catalog number selected: M5001

Catalog number selected: M5005

Catalog number selected:  M5006

Catalog number selected: M5008

Robust Hot Start PCR with Convenient Room-Temperature Setup

 

High-Performance Hot-Start PCR

GoTaq® Hot Start Polymerase contains high-performance GoTaq® DNA Polymerase bound to a proprietary antibody that blocks polymerase activity. The polymerase activity is restored during the initial denaturation step when the amplification reactions are heated at 94–95°C for two minutes. This enables hot-start PCR, where polymerase activity is eliminated or minimized at temperatures below 70°C. GoTaq® Hot Start Polymerase also exhibits 5´→3´ exonuclease activity. The enzyme is supplied with a tube of 25mM MgCl2 to optimize the magnesium concentration in your reactions.

GoTaq® Hot Start Polymerase is supplied with 5X Green GoTaq® Flexi Buffer and 5X Colorless GoTaq® Flexi Buffer. The buffers contain a compound that increases sample density so that samples sink easily into wells of an agarose gel. The green buffer also contains two dyes (yellow and blue) that separate to allow easy monitoring during electrophoresis. Use the green reaction buffer for direct-to-gel analysis after amplification and the colorless reaction buffer for amplifications where the dyes may interfere with post-amplification analysis such as fluorescence or absorbance testing.

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Performance Guarantee: Promega PCR systems, enzymes and reagents are proven in PCR to ensure reliable, high-performance results. If you are not completely satisfied with any Promega PCR product, we will send a replacement or refund your account.

GoTaq® Hot Start Polymerase with Colorless Flexi Buffer (C) and with Green Flexi Buffer (G) outperforms antibody (I) or chemically modified (A) competitor hot-start DNA polymerases for amplification of a 2.4kb fragment of the Human APC gene.

Protocols

Complete Protocol

GoTaq® Hot Start Polymerase Product Information

[easy_media_download url=”https://gomolecular.pk/wp-content/uploads/2021/06/GoTaq-Hot-Start-Polymerase-9PIM500.zip” text=”Download” color=”blue_three” ]

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What’s in the box?

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SDS For M5001

[easy_media_download url=”https://gomolecular.pk/wp-content/uploads/2021/06/M5001.zip” text=”Download SDS” color=”blue_three” ]

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

What’s in the box?

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SDS For M5005

[easy_media_download url=”https://gomolecular.pk/wp-content/uploads/2021/06/M5005.zip” text=”Download SDS” color=”blue_three” ]

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

What’s in the box?

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SDS For M5006

[easy_media_download url=”https://gomolecular.pk/wp-content/uploads/2021/06/M5006.zip” text=”Download SDS” color=”blue_three” ]

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

What’s in the box?

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SDS For M5008

[easy_media_download url=”https://gomolecular.pk/wp-content/uploads/2021/06/M5008.zip” text=”Download SDS” color=”blue_three” ]

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

Patent and Disclaimers

Use of this product for basic PCR is outside of any valid US or European patents assigned to Hoffman La-Roche or Applera. This product can be used for basic PCR in research without any license or royalty fees. This product is for research use only.