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GoTaq® Hot Start Master Mixes

Hot-Start GoTaq® DNA Polymerase in a Ready-to-Use Master Mix

  • All-in-one master mix; just add template and primers
  • Minimize nonspecific amplification and primer-dimers
  • Use the green master mix for direct-to-gel analysis, and use the colorless master mix when post-amplification fluorescence or absorbance analysis is required

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Description

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Size:

100 reactions: Catalog number selected:  M5132

1,000 reactions: Catalog number selected: M5133

Size:

100 reactions: Catalog number selected: M5122

1000 reactions: Catalog number selected: M5123

 

Hot-Start GoTaq® DNA Polymerase in a Ready-to-Use Master Mix

 

 

GoTaq® Hot Start Polymerase contains the high-performance GoTaq® DNA Polymerase bound to a proprietary antibody that blocks polymerase activity. The polymerase activity is restored during the initial denaturation step when the amplification reactions are heated at 94-95 degrees C for two minutes. This enables hot-start PCR, where polymerase activity is eliminated or minimized at temperatures below 70 degrees C. GoTaq® Hot Start Polymerase exhibits 5′ –> 3′ exonuclease activity.

GoTaq® Hot Start Master Mixes are premixed, ready-to-use solutions containing GoTaq® Hot Start Polymerase, magnesium, dNTPs and buffer. Reactions can be set up in less than a minute at room temperature; simply add your template, water and primers. The Master Mixes contain a compound that increases sample density so that samples sink easily into wells of an agarose gel. The GoTaq® Hot Start Green Master Mix also contains two dyes (yellow and blue) that separate to allow easy monitoring during electrophoresis. Use the Green Master Mix for direct-to-gel analysis after amplification and the Colorless Master Mix for amplifications where the dyes may interfere with post-amplification analysis such as fluorescence or absorbance testing.

Protocols

Complete Protocol

GoTaq® Hot Start Green Master Mix Protocol

[easy_media_download url=”https://gomolecular.pk/wp-content/uploads/2021/06/GoTaq-Hot-Start-Green-Master-Mix-9PIM512.zip” text=”Download” color=”blue_three” ] 

GoTaq® Hot Start Colorless Master Mix Protocol

[easy_media_download url=”https://gomolecular.pk/wp-content/uploads/2021/06/GoTaq-Hot-Start-Colorless-Master-Mix-9PIM513.zip” text=”Download” color=”blue_three” ]

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What’s in the box?

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SDS For M5132

[easy_media_download url=”https://gomolecular.pk/wp-content/uploads/2021/06/M5132.zip” text=”Download SDS” color=”blue_three” ] 

SDS For M5133

[easy_media_download url=”https://gomolecular.pk/wp-content/uploads/2021/06/M5133.zip” text=”Download SDS” color=”blue_three” ]

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

 

Storage Conditions

What’s in the box?

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SDS For M5122

[easy_media_download url=”https://gomolecular.pk/wp-content/uploads/2021/06/M5122.zip” text=”Download SDS” color=”blue_three” ] 

SDS For M5123

[easy_media_download url=”https://gomolecular.pk/wp-content/uploads/2021/06/M5123.zip” text=”Download SDS” color=”blue_three” ]

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

Patent and Disclaimers

Use of this product for basic PCR is outside of any valid US or European patents assigned to Hoffman La-Roche or Applera. This product can be used for basic PCR in research without any license or royalty fees. This product is for research use only.