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GoTaq® G2 Hot Start Taq Polymerase

High-Performance Hot Start Taq with Convenient Room-Temperature PCR Setup

  • Hot-start Taq eliminates nonspecific PCR amplification
  • Detects low-copy-number targets
  • Allows optimization of reactions with Flexi Reaction Buffer and separate MgCl2
  • Supplied at a concentration of 5u/μl

 

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Description

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Catalog number selected: M7401

Catalog number selected: M7405

Catalog number selected: M7406

Catalog number selected: M7408

High-Performance Hot Start Taq with Convenient Room-Temperature PCR Setup

 

Hot Start Taq with Superior Performance, Improved Yield, Greater Sensitivity

GoTaq® G2 Hot Start Taq is the second generation of GoTaq® products. It comes in a variety of formats designed to provide maximum flexibility, control and convenience.

GoTaq® G2 Hot Start Taq is bound to a proprietary antibody that blocks polymerase activity until the reaction is heated to 94–95°C during initial denaturation. Polymerase activity is inhibited at temperatures below 70°C, allowing convenient, room-temperature reaction setup. Hot-start Taq is advantageous for some amplification targets because it may eliminate or minimize formation of primer-dimer or nonspecific products.  In some cases, hot-start PCR may improve yields.

GoTaq® G2 Hot Start Taq is available as a master mix or as a standalone enzyme, it is supplied with 5X Green GoTaq® Flexi Buffer, 5X Colorless GoTaq® Flexi Buffer and 25mM MgCl2. GoTaq® G2 Hot Start Polymerase exhibits 5´→3´ exonuclease activity.

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Performance Guarantee: Promega PCR systems, enzymes and reagents are proven in PCR to ensure reliable, high-performance results. If you are not completely satisfied with any Promega PCR product, we will send a replacement or refund your account.

GoTaq® G2 Hot Start Taq exhibits higher yields and greater detection sensitivity.

The 2.2kb APC target was amplified from decreasing amounts of input gDNA (33ng, 3.3ng and 330pg, left to right for each enzyme) using GoTaq® G2 Hot Start Taq with Green (G) and Colorless (C) buffers and the leading competitors (L1, R, Q, Q+, L2 and L3) according to manufacturers’ recommendations.

Protocols

Complete Protocol

GoTaq® G2 Hot Start Polymerase Product Information

 

[easy_media_download url=”https://gomolecular.pk/wp-content/uploads/2021/06/GoTaq-G2-Hot-Start-Polymerase-9PIM740-.zip” text=”Download” color=”blue_three” ]

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What’s in the box?

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SDS For M7401

[easy_media_download url=”https://gomolecular.pk/wp-content/uploads/2021/06/M7401.zip” text=”Download SDS” color=”blue_three” ]

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures

Storage Conditions

What’s in the box?

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SDS For M7405

[easy_media_download url=”https://gomolecular.pk/wp-content/uploads/2021/06/M7405.zip” text=”Download SDS” color=”blue_three” ]

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

What’s in the box?

[wpdatatable id=9 table_view=regular]

SDS For M74016

[easy_media_download url=”https://gomolecular.pk/wp-content/uploads/2021/06/M7406.zip” text=”Download SDS” color=”blue_three” ] 

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

What’s in the box?

[wpdatatable id=10 table_view=regular]

SDS For M7408

[easy_media_download url=”https://gomolecular.pk/wp-content/uploads/2021/06/M7408.zip” text=”Download SDS” color=”blue_three” ] 

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

Patent and Disclaimers

Use of this product for basic PCR is outside of any valid US or European patents assigned to Hoffman La-Roche or Applera. This product can be used for basic PCR in research without any license or royalty fees. This product is for research use only