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GoTaq® DNA Polymerase

Taq DNA Polymerase with Buffers for Enhanced PCR Amplification

  • Direct-to-gel convenience
  • Can be used with your current PCR cycling conditions
  • Taq polymerase supplied at a concentration of 5u/μ

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Description

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Catalog number selected: M3001

Catalog number selected: M3005

Catalog number selected: M3008

Taq DNA Polymerase with Buffers for Enhanced PCR Amplification

 

Taq Polymerase for Robust PCR with and Direct-to-Gel Convenience

GoTaq® DNA Polymerase is a proprietary formulation of Taq polymerase that gives robust amplification equal to and, in some cases, superior to that of standard Taq.

The 5X GoTaq® Green and Colorless Reaction Buffers supplied with GoTaq® DNA Polymerase contain MgCl2 at a concentration of 7.5mM for a final concentration of 1.5mM in the 1X reaction.

The 5X Green and 5X Colorless Reaction Buffers allow you to go directly from thermal cycler to gel analysis. These buffers contain a compound that increases sample density, so that samples sink easily into wells of an agarose gel. The green buffer also contains two dyes (yellow and blue) that separate to allow easy monitoring during electrophoresis. The blue dye comigrates with 3–5kb DNA fragments in a 1% agarose gel. The yellow dye migrates ahead of primers (<50bp). Use the green reaction buffer for direct-to-gel analysis after amplification and the colorless reaction buffer for post-amplification analysis by fluorescence or absorbance without prior DNA purification. PCR enhancers, such as betaine and DMSO, have no effect on the color or characteristics of the GoTaq® Green Reaction Buffer.

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Performance Guarantee:
 Promega PCR systems, enzymes and reagents are proven in PCR to ensure reliable, high-performance results. If you are not completely satisfied with any Promega PCR product, we will send a replacement or refund your account.

Protocols

Complete Protocol

GoTaq® DNA Polymerase (M300) Protocol
[easy_media_download url=”https://gomolecular.pk/wp-content/uploads/2021/06/GoTaq-DNA-Polymerase-9PIM300.zip” text=”Download” color=”blue_three” ]
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What’s in the box?

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SDS For M3001

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Use Restrictions

For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.

Storage Conditions

What’s in the box?

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SDS For M3005

[easy_media_download url=”https://gomolecular.pk/wp-content/uploads/2021/06/M3005.zip” text=”Download SDS” color=”blue_three” ]

Use Restrictions

For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.

Storage Conditions

What’s in the box?

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SDS For M3008

[easy_media_download url=”https://gomolecular.pk/wp-content/uploads/2021/06/M3008.zip” text=”Download SDS” color=”blue_three” ]

Use Restrictions

For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated

Storage Conditions

Patent and Disclaimers

Use of this product for basic PCR is outside of any valid US patents assigned to Hoffman La-Roche or Applera. This product can be used for basic PCR in research, commercial or diagnostic applications without any license or royalty fees.