Molecular Products Co.

Search
customer-support

Call Us Now:

0334-7752577

GoScript™ Reverse Transcriptase

Reliable cDNA Synthesis for Sensitive Detection of Expression

  • Available as a standalone enzyme, a complete reverse transcription kit or as a master mix with Oligo(dT) or Random Primers
  • Sensitive transcription of both high-copy and low-copy messages
  • Transcribes short and long transcripts; processes through secondary structure

Please Enquire

Description

[ts-heading h2=”Choose a system” css_animation=”fadeInUp”][/ts-heading]

Size:

100 reactions : Catalog number selected: A5003

500 reactions: Catalog number selected: A5004

 

Size:

50 reactions : Catalog number selected: A5000

100 reactions: Catalog number selected: A5001

 

Size:

50 reactions : Catalog number selected: A2790

500 reactions: Catalog number selected: A2791

 

Size:

50 reactions : Catalog number selected: A2800

500 reactions: Catalog number selected: A2801

 

[ts-heading h2=”

Sensitive Reverse Transcriptase Ideal for Challenging Templates

” txt_align=”center” reverse_heading=”” css_animation=”bounceInUp”]GoScript™ Reverse Transcriptase utilizes M-MLV reverse transcriptase enzyme and state-of-the-art buffer to drive robust, reliable cDNA synthesis of a full range of rare and abundant transcripts, even with difficult templates and in the presence of PCR inhibitors.[/ts-heading]

Resilient

Synthesizes cDNA in the presence of strong PCR inhibitors

Sensitive

Transcribes both high copy and low copy messages

Reliable

Processes through secondary structure

Processive

Transcribes short and long transcripts

[ts-heading h2=”One Reverse Transcriptase, Four Convenient Formats” css_animation=”fadeInDown”]GoScript™ Reverse Transcriptase is available in convenient mixes with either Oligo (dT) primers or random primers, as part of a complete kit, and as a stand-alone enzyme.[/ts-heading]

GoScript™ Reverse Transcription System

Includes GoScript™ Reverse Transcriptase, Reaction Buffer, MgCl2, dNTP Mix, Oligo(dT), Random Primers and Recombinant RNasin® Ribonuclease Inhibitor, and is designed for efficient synthesis of first-strand cDNA. The system components can effectively reverse transcribe RNA templates starting with total RNA, poly(A)+ mRNA or synthetic transcript RNA.

GoScript™ Reverse Transcription Mix, Oligo(dT)

GoScript™ Reverse Transcription Mix, Oligo(dT) includes an optimized set of reagents designed for efficient synthesis of first-strand cDNA. The Oligo(dT) priming initiates first-strand synthesis by annealing to the 3’ end of any polyadenylated RNA molecule. Reverse Transcription of total RNA or mRNA will yield oligo(dT)-primed products from any poly(A)+RNA. The population of cDNAs produced using this method allows many different downstream analyses from a single reverse transcription reaction.

GoScript™ Reverse Transcription Mix, Random Primers

Includes random primers for synthesis of first-strand cDNA in preparation for qPCR amplification. Priming using random primers is the most generally used method of initiating cDNA synthesis from a variety of RNA templates. Random primers can be used to prime first-strand cDNA synthesis from all RNA molecules, including those that do not possess a poly(A)+ tail and RNA isolated from prokaryotic sources.

GoScript™ Reverse Transcriptase

Includes GoScript™ M-MLV reverse transcriptase enzyme and 5X GoScript™ Reaction Buffer.

[ts-heading h2=”Reverse Transcriptase Performance Data” txt_align=”center” css_animation=”fadeInUp”]

The data presented below illustrate the sensitivity, inhibitor resistance, and performance of GoScript™ Reverse transcriptase in cDNA Synthesis and RT-qPCR.

[/ts-heading]

Efficient cDNA Synthesis Over a Wide Range of Input RNA

Direct quantification of six different targets in human total RNA using GoTaq® 2-Step RT-qPCR System. Nine parallel large-scale GoScript™ RT reactions were performed, containing log-fold variations of input human reference total RNA (10ng/µl to 0.1fg/µl). 10µl fractions of each reverse transcription reaction product, representing 100ng to 1fg of input RNA, were sampled directly into triplicate GoTaq® qPCR reactions, without dilution, for six separate monoplex assays. Each assay was designed to be specific for a different RNA target with an anticipated, proportionally different expression level: extremely high (5.8S rRNA), high (ACTB and B2M), moderate (HPRT ) or low (RPL29 and TBP1 ). In each different model, the results demonstrate the desired ≅3.3 cycle change per Δlog input, but with different Cq’s reflecting relative proportion within the total RNA sample.

Resistance to Common Inhibitors

An in vitro-transcribed RNA was reverse transcribed using oligo(dT) primer according to the manufacturer’s instructions. Reverse transcription was carried out in the absence and presence of ethanol (5%, 10% and 20%). cDNA was analyzed by qPCR using GoTaq® qPCR Master Mix. Changes in cycle threshold values in the absence and presence of ethanol were determined and converted to relative change in transcript level = (2Cqinhibitor – Cqnone). Data are the average of three separate experiments.

[ts-heading h2=”Efficient Detection of Low-Copy Targets” css_animation=”fadeInUp”]Here, a series of 10 fold dilutions (1µg – 100pg) of total human RNA (Agilent, Cat #750500) was reverse transcribed using the GoScript™ Reverse Transcription Mix, Random Primers or Oligo(dT). The resulting cDNA was evaluated by qPCR amplification of the low-copy genes, APC or TBP, using the GoTaq® qPCR Master Mix (Cat. # A6002). Standard curves show that the primers had a calculated efficiency of 99% (APC), and 97% (TBP). Both values are within the range recommended by the MIQE guidelines. No-reverse transcriptase and no-template controls gave negative results.[/ts-heading]

GoScript™ RT Mix Oligo(dT), TBP Variable RNA Input

GoScript™ RT Mix, Random Primers, TBP Variable RNA Input

[ts-heading h2=”Sensitivity and Specificity” txt_align=”center” css_animation=”fadeInUp”]GoScript™ Reverse Transcriptase used in combination with GoTaq® qPCR Master Mix can provide detection of input templates over a 9-log order dynamic range.[/ts-heading]

GoScript™ Reverse Transcriptase cDNA synthesis coupled with GoTaq® qPCR Master Mix can detect input template over a 9-log order dynamic range. Panel A. Ten RNA template mixes were assembled to model a series of tenfold differences of a specific RNA abundance (1 × 102 to 1 × 1011 copies) in a constant mass of 10ng of human total RNA. cDNAs for each RNA sample were prepared using GoScript™ Reverse Transcriptase and amplified using GoTaq® qPCR at each level of total cDNA (n=3 each point). Inset (Panel A) shows dissociation profile for the 50-, 500- and 5,000-copy standard and 5-copy detected levels of MS2 + cDNA and NTC reactions, demonstrating specificity. Standard curve shown in Panel B illustrates the quantitation and detection of the “unknown” samples. ■ = tenfold standard dilutions; x = detection of 5-copy samples.

[ts-custom-heading text=”Protocols” font_container=”tag:h1|text_align:left” css_animation=”fadeInDown”]

Complete Protocol

GoScript™ Reverse Transcriptase Product Information

[easy_media_download url=”https://gomolecular.pk/wp-content/uploads/2021/06/GoScript-Reverse-Transcriptase-9PIA500.zip” text=”Download” color=”blue_three” ]

GoScript™ Reverse Transcription System Technical Manual

[easy_media_download url=”https://gomolecular.pk/wp-content/uploads/2021/06/GoScript-Reverse-Transcription-System-TM316.zip” text=”Download” color=”blue_three” ]

GoScript™ Reverse Transcription Mix, Oligo(dT)

[easy_media_download url=”https://gomolecular.pk/wp-content/uploads/2021/06/9PIA279-GoScript-Reverse-Transcription-MixOligodT.zip” text=”Download” color=”blue_three” ]

GoScript™ Reverse Transcription Mix, Random Primers

[easy_media_download url=”https://gomolecular.pk/wp-content/uploads/2021/06/9PIA280-GoScript-Reverse-Transcription-Mix_Random-Primers.zip” text=”Download” color=”blue_three” ]

Quick Protocols

GoScript™ Reverse Transcription System Quick Protocol FB114

[easy_media_download url=”https://gomolecular.pk/wp-content/uploads/2021/06/GoScript™-Reverse-Transcription-System-Quick-Protocol-FB114.pdf” text=”Download” color=”blue_three” ]

 

[ts-heading h2=”Specifications” txt_align=”center” css_animation=”fadeInUp”][/ts-heading]

What’s in the box?

[wpdatatable id=97 table_view=regular]

SDS For  A5003
[easy_media_download url=”https://gomolecular.pk/wp-content/uploads/2021/06/A5003.zip” text=”Download SDS” color=”blue_three” ]

 

SDS For A5004
[easy_media_download url=”https://gomolecular.pk/wp-content/uploads/2021/06/A5004.zip” text=”Download SDS” color=”blue_three” ]

Use Restrictions

For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.

Storage Conditions

What’s in the box?

[wpdatatable id=98 table_view=regular]

SDS For A5000
[easy_media_download url=”https://gomolecular.pk/wp-content/uploads/2021/06/A5000.zip” text=”Download SDS” color=”blue_three” ]

SDS For A5001
[easy_media_download url=”https://gomolecular.pk/wp-content/uploads/2021/06/A5001.zip” text=”Download SDS” color=”blue_three” ]ّ

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

What’s in the box?

[wpdatatable id=99 table_view=regular]

SDS For A2790
[easy_media_download url=”https://gomolecular.pk/wp-content/uploads/2021/06/A2790.zip” text=”Download SDS” color=”blue_three” ]

SDS For A2791
[easy_media_download url=”https://gomolecular.pk/wp-content/uploads/2021/06/A2791.zip” text=”Download SDS” color=”blue_three” ]

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

What’s in the box?

[wpdatatable id=100 table_view=regular]

SDS For A2800
[easy_media_download url=”https://gomolecular.pk/wp-content/uploads/2021/06/A2800.zip” text=”Download SDS” color=”blue_three” ]

SDS For A2801
[easy_media_download url=”https://gomolecular.pk/wp-content/uploads/2021/06/A2801.zip” text=”Download SDS” color=”blue_three” ]

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions