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Direct PCR: XpressAmp™ Direct Amplification Reagents

Rapid, Extraction-Free Viral Sample Preparation for RT-qPCR Amplification

  • Ten-minute, room-temperature sample lysis
  • Easily automatable for high-throughput needs
  • Use Restrictions: For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.

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Description

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Catalog number selected: A8882

Catalog number selected: A8880

Direct Amplification for Rapid PCR Results

The XpressAmp™ Direct Amplification Reagents provide a fast, RNA extraction-free method to prepare viral samples for PCR-based amplification using commonly available RT-qPCR reagents. Collect the samples by nasopharyngeal swab in universal or viral transport media, and perform direct amplification analysis in RT-qPCR. The simple sample preparation method requires only a 10-minute, room-temperature incubation that is easy to automate.

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Collect and Store Sample
Use commonly available nasopharyngeal swabs stored in transport media.

Lyse Sample
Mix sample and XpressAmp™ Lysis Buffer (1:1). Incubate for 10 minutes at room temperature.

Amplify and Analyze
Add lysed sample to RT-qPCR amplification containing XpressAmp™ Solution.

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XpressAmp™ Detection of RNA from Inactivated Influenza/RSV Virus Pellet

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Viral transport media (VTM) was inoculated with a nasopharyngeal swab and spiked with RSV A and Influenza B (Hong Kong) virus reconstituted from Helix Elite™ Inactivated Standard, Inactivated Influenza A/B and Respiratory Syncytial Virus. This high-level virus sample (1 × 103 copies/μl) was diluted 1:10 and 1:100 in VTM to create the medium- and low-level virus samples. In parallel, two users created sample lysates from the spiked VTM samples using the XpressAmp™ Direct Amplification Reagents. Both users then detected the presence of RSV A and Influenza B by RT-qPCR using GoTaq® Probe 1-Step qPCR System (Cat.# A6121) supplemented with the XpressAmp™ Solution. N=6.

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Viral transport media (VTM) was inoculated with a nasopharyngeal swab and spiked with Synthetic SARS-CoV-2 RNA Control 2 (Twist Biosciences, Cat.# 102024, final concentration 1 × 104 copies/μl). Spiked VTM samples were lysed by combining 5μl of sample with 5μl of prepared XpressAmp™ Lysis Buffer and incubated at room temperature for 10 minutes. Following incubation, 5μl of sample lysate was added to a monoplex GoTaq® Probe 1-Step RT-qPCR (25μl) containing XpressAmp™ Solution and amplified using the 2019 nCoV RUO kit (IDT, Cat.# 10006713) and thermal cycled according to the CDC protocol. N=8 amplification replicates.

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An independent laboratory performed an evaluation of the ability of the XpressAmp™ Lysis Buffer, used to prepare viral sample for RT-qPCR analysis, to inactivate the SARS-CoV-2 virus.

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SARS-CoV-2 Viral Inactivation White Paper

[easy_media_download url=”https://gomolecular.pk/wp-content/uploads/2021/06/Inactivation-of-SARS-CoV-2-Virus-by-XpressAmp-White-Paper-WP118.pdf” text=”Read PDF” color=”blue_three” target=”new” ]

Protocols

Complete Protocol

XpressAmp™ Direct Amplification Reagents Technical Manual

[easy_media_download url=”https://gomolecular.pk/wp-content/uploads/2021/06/XpressAmp-Direct-Amplification-Reagents-Technical-Manual-TM647.zip” text=”Download” color=”blue_three” ]

 

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What’s in the box?

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SDS For A8882

[easy_media_download url=”https://gomolecular.pk/wp-content/uploads/2021/06/a8882.zip” text=”Download SDS” color=”blue_three” ]

Use Restrictions

For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.

Storage Conditions

What’s in the box?

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SDS For  A8880

[easy_media_download url=”https://gomolecular.pk/wp-content/uploads/2021/06/a8880.zip” text=”Download SDS” color=”blue_three” ]

Use Restrictions

For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.

Storage Conditions