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DdeI

Capable of digesting DNA in 15 minutes or less

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Description

10μ/μl

Catalog number selected: R6291

200u

Catalog number selected: R6291

1,000u

Catalog number selected: R6295

Cut Site:

CTNA  G
G  ANTC

Notes:

  1. For site-specific methylation information, consult McClelland, M., Nelson, M. and Raschke, E. (1994) Nucl. Acids Res. 22, 3640–59.
  2. DdeI produces an ambiguous extension, which is difficult to ligate.

Incubation Conditions: Buffer D. 37°C.

Source: Desulfovibrio desulfuricans.

Storage Buffer: 10mM Tris-HCl (pH 7.4), 50mM NaCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 0.01% Triton® X-100 and 50% glycerol.

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Protocols

Complete Protocol

 

 Restriction Enzyme Protocol

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What’s in the box?

You are viewing: R6291 Change Configuration

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

What’s in the box?

You are viewing: R6295 Change Configuration

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SDS

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions