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BclI

Genome qualified to ensure optimal performance in genomic analysis applications

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Description

10μ/μl

Catalog number selected: R6651

100u

Catalog number selected: R6651

Cut Site:

TGATC  A
A  CTAGT

Notes:

  1. Sensitive to dam methylation.
  2. For site-specific methylation information, consult McClelland, M., Nelson, M. and Raschke, E. (1994) Nucl. Acids Res. 22, 3640–59.
  3. Star activity may be observed with glycerol concentrations >12% or with enzyme:DNA ratios >25u/μg.
  4. Ends generated with BclI can be directly ligated to ends generated with BglII, BamHI or XhoII. These restriction sites may not be regenerated in the ligation product.

Incubation Conditions: Buffer C. 50°C. (BclI exhibits 10–25% activity at 37°C.)

Source: Bacillus caldolyticus.

Storage Buffer: 10mM Tris-HCl (pH 7.4), 50mM NaCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.

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Protocols

Complete Protocol

 

 Restriction Enzyme Protocol

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What’s in the box?

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions