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BamHI

  • Capable of digesting DNA in 15 minutes or less
  • Active and capable of digestion directly in GoTaq® Green Master Mix
  • Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications
  • Available at high concentration, containing 12,500 units of BamHI at a concentration of 40–80u/μl

Please Enquire

Description

10u/μl

Catalog number selected: R6021

2,500u

Catalog number selected: R6021

12,500u

Catalog number selected: R6025

40-80u/μl

Catalog number selected: R4024

12,500u

Catalog number selected: R4024

Cut Site:

GGATC  C
C   CTAGG

Usage Notes:

  1. Star activity may be observed with glycerol concentrations >12%, enzyme:DNA ratio >25u/μg, low salt conditions or in the presence of Mn2+.
  2. Ends generated with BamHI can be directly ligated to ends generated with BglII, BclI and XhoII. These restriction sites are not regenerated in the ligation product.

Incubation Conditions: Buffer E. 37°C.

Source: Bacillus amyloliquefaciens H.

Storage Buffer: 10mM Tris-HCl (pH 7.4), 300mM KCl, 5mM MgCl2, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.

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Protocols

Complete Protocol

 

 Restriction Enzyme Protocol

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What’s in the box?

You are viewing: R6021 Change Configuration

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

What’s in the box?

You are viewing: R6025 & R6045 Change Configuration

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SDS

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions