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AMV Reverse Transcriptase

Efficient Reverse Transcription of Templates with High Secondary Structure

  • DNA polymerization using template DNA, RNA or RNA:DNA hybrids
  • Requires a primer as well as magnesium or manganese
  • Stable at higher reaction temperatures (37–58°C)
  • Supplied at a concentration of 10u/μl (Cat.# M5101, M5108) or 20–25u/µl (Cat.# M9004)

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Description

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Catalog number selected:  M5101

Catalog number selected: M5108

Catalog number selected: M9004

Efficient Reverse Transcription of Templates with High Secondary Structure

 

Reverse Transcriptase with Stability at Higher Temperatures

Avian Myeloblastosis Virus Reverse Transcriptase (AMV RT) catalyzes DNA polymerization using template DNA, RNA or RNA:DNA hybrids. It requires a primer (DNA primers are more efficient than RNA primers) as well as Mg2+ or Mn2+. The enzyme possesses an intrinsic RNase H activity. Both nonionic detergents and sulfhydryl compounds stabilize the enzyme activity in vitro.

Features

  • High Concentration: Cat.# M9004 contains 600 units of AMV RT at 20–25u/μl.
  • 5X Reaction Buffer: 250mM Tris-HCl (pH 8.3 at 25°C), 250mM KCl, 50mM MgCl2, 2.5mM spermidine, 50mM DTT.
  • Temperature Stability: AMV RT is the preferred reverse transcriptase for templates with high secondary structure due to its stability at higher reaction temperatures (37–58°C).

Applications

  • First- and second-strand synthesis of cDNA.
  • Primer extensions and RNA sequencing.
  • RT-PCR. Up to 10μl of an RT reaction containing AMV RT and the supplied AMV RT Reaction Buffer can be added to a 50μl PCR amplification reaction that uses Taq DNA polymerase. If GoTaq® DNA Polymerase or PCR Master Mix are used, up to 25μl of an RT reaction can be added per 50μl PCR.

Further Reading

  1. Kacian, D.L. (1977) Meth. Virol. 6, 143.
  2. Mierendorf, R.C. and Pfeffer, D. (1987) Meth. Enzymol. 152, 563–6.
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Protocols

Complete Protocol

AMV Reverse Transcriptase Protocol
[easy_media_download url=”https://gomolecular.pk/wp-content/uploads/2021/06/AMV-Reverse-Transcriptase-9PIM510.zip” text=”Download” color=”blue_three” ]
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What’s in the box?

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SDS For M5101

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Use Restrictions

For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.

Storage Conditions

What’s in the box?

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SDS For  M5108

[easy_media_download url=”https://gomolecular.pk/wp-content/uploads/2021/06/M5108.zip” text=”Download SDS” color=”blue_three” ]

Use Restrictions

For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.

Storage Conditions

What’s in the box?

[wpdatatable id=85 table_view=regular]

SDS For M9004

[easy_media_download url=”https://gomolecular.pk/wp-content/uploads/2021/06/M9004.zip” text=”Download SDS” color=”blue_three” ]

Use Restrictions

For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.

Storage Conditions