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4-CORE® Buffer Pack

Aliquots of 10X Restriction Enzyme Buffers A, B, C and D

  • Majority of Promega restriction enzymes have optimal activity in one of these four 10X reaction buffers

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Description

4ml

Catalog number selected: R9921

The 4-CORE® Buffer Pack contains convenient aliquots of Promega Restriction Enzyme 10X Buffers A, B, C and D. The majority of Promega restriction enzymes have optimal activity in one of these four 10X reaction buffers.

See the table of Promega 1X Restriction Enzyme Buffer Composition below.

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MULTI-CORE™ Buffer (1X) = 25mM Tris-acetate (pH 7.5 at 37°C), 100mM potassium acetate, 10mM magnesium acetate, 1mM DTT.

Notes:

  1. For each 10°C rise in temperature between 0°C and 25°C, the pH of Tris buffers decreases 0.31 pH units.
  2. For each 10°C rise in temperature between 25°C and 37°C, the pH of Tris buffers decreases 0.25 pH units.
  3. All restriction enzymes are supplied with 10mg/ml Acetylated BSA. Although BSA is not absolutely required for activity, it has been shown to enhance activity of many restriction enzymes. We recommend adding BSA to all restriction digests at a final concentration of 0.1mg/ml.
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What’s in the box?

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions