Cloning your gene can be one of the most difficult parts of an experiment. The first step is to decide which method will be most effective for inserting your gene precisely at the target locus.
PCR Cloning Guide
Jump into the PCR Cloning section of our guide to subcloning and learn about the techniques and products you’ll need to be successful.
A Quick Method for A-Tailing PCR Products
Explore a few strategies you might not have considered to overcome the challenges of “A” overhangs.
Cloning with pGEM-T Vectors: Ligation
Learn how to ligate your blunt-ended DNA fragments using pGEM-T vectors.
T-Vector Cloning FAQ
Need more tips for successful cloning? Check out these answers to frequently asked questions.
Cloning Modified Blunt-Ended DNA Fragments into T-Vectors
Read about a method for eliminating some of the requirements of conventional blunt-end cloning.
Restriction Enzyme Cloning
Tips for Restriction Enzyme-Digested Vectors and Inserts
Read a few useful tips from some restriction enzyme experts.
Restriction Enzyme Digestion Applications and Resources
Explore applications for restriction enzyme digests and more in this resource to help you conquer your research.
Restriction Enzyme Tool
Incubation temperature, recognition sequence, overhang sequence—get all the info you need about your restriction enzyme.